Preparation of Polyclonal Antibody against ZmBT1 Protein and Its Application in Hormone-Regulated Starch Synthesis
نویسندگان
چکیده
In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze expression distribution various tissues, we prepared a polyclonal antibody. Specifically, successfully expressed recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) purified Gst-ZmBT1-C as antigen for antibody preparation. Our results confirmed that protein tissues can be specifically recognized by Through Western blotting, observed varied with highest content endosperm. Furthermore, employed combination blotting quantitative real-time PCR show level influenced plant hormones. This finding suggests plays critical opens up new avenues functional studies this protein.
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ژورنال
عنوان ژورنال: Agronomy
سال: 2023
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy13071805